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R
developed by Michael Brattain.
RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor (h-TRbeta1). The level of p53 protein is higher in RKO (ATCC CRL-2577) cells than in RKO-E6 (ATCC CRL-2578) cells. [53331]
The RKO cell line is the parental cell line (isogenic) of RKO-E6 (ATCC CRL-2578) and RKO-A545-1 (ATCC CRL-2579). It can be used as the control cell line for investigating the effects of p53 and gadd45 on cellular parameters.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
